Production of recombinant proteins requires the easy culturing, fast expression and high yields, however, one major problem is the insoluble expression in E.coli expression system. Maltose binding protein (MBP) tag is usually applied as an useful protein solubility partner to enhance the solubility of recombinant protein much more effective than GST fusion tag and TRx fusion tag.
MBP Tag
MBP is anaturally occurring protein encoded by the malE gene, which is about 42 kDa in molecular weight. The major responsibilities of MBP are to uptake, breakdown and transport maltodextrin. MBP tag is mostly fused to the N-terminus to increase recombinant soluble expression. Creative Biostructure has developed combinatorial-tagging methods for custom needs. For example, using MBP tag as solubility-enhancing tag combined with a GST tag or His tag as the purification tag.
Advantages of MBP-tagged Proteins:
1. MBP-tagged proteins are usually able to achieve high yields in the cytoplasm of E. coli since the juxtaposition leads to efficient translation initiation.
2. MBP tag can be used to keep its passenger proteins from proteolytic degradation.
3. MBP tag is a natural affinity tag, which can be used to enhance purification of the passenger proteins.
4. MBP-tagged proteins are with remarkably enhanced solubility.
Creative Biostructure has devoted to MBP-tagged protein expression and purification for years. We are happy to accelerate your project by our resourceful and professional technical support platform. Please see the related services for more information and feel free to Contact Us.
